The Short Answer: Yes, But With Serious Caveats
Yes, your business can absolutely have more than one line of credit. There is no federal law or universal banking rule that limits you to a single line. Many established businesses strategically use multiple lines of credit from different lenders to manage cash flow, fund separate projects, or create a financial safety net.
However, for a small or new business, getting approved for a second or third line of credit is more complex than getting the first. Each new lender will conduct a thorough underwriting process. They will look at your existing debt obligations—including any current lines of credit—to determine if your business can safely handle more debt. They are primarily concerned with your ability to repay.
Lenders view multiple lines of credit as a potential risk indicator, a practice sometimes called "credit stacking." They will scrutinize your business's revenue, cash flow, time in business, and both your business and personal credit history. For business owners who are new and may not qualify with traditional banks, alternative lenders might be an option, but often come with higher interest rates and stricter terms. The core question for any lender remains the same: does your business generate enough consistent income to service all its debts, including the new one you're applying for? Answering this question for yourself before you apply is the most critical first step.